Genome-wide association study of in vitro drug resistance in Kenya

As a part of clinical studies being undertaken by the KEMRI-Wellcome Trust Research Programme, samples were collected in the Kilifi district of Kenya. Alexis Nzila and his colleagues adapted these field isolates to laboratory culture and tested their in vitro sensitivity to antimalarial drugs including lumefantrine, chloroquine, piperaquine and dihydroartemisinin (DHA). The phenotypic data are now being used in genome-wide association studies with parasite genotypes generated by Illumina sequencing to identify regions associated to decreased sensitivity to the antimalarial drugs.

Summary

Samples/Year

QC Pass

Samples that passed Whole-Genome Sequencing Quality Control (QC)

Resistance

Predicted resistance status for main antimalarial drug treatments from molecular markers

Locations

Kilifi

Samples/Year

QC Pass

Samples that passed Whole-Genome Sequencing Quality Control (QC)

Resistance

Predicted resistance status for main antimalarial drug treatments from molecular markers